Rossana Bertini obtained her diploma in Lyric Art at the Conservatorio Francesco Morlacchi  (Perugia).

She has performed with some of the foremost early music groups of Europe, including Europa Galante (Fabio Biondi), Concerto Italiano (Rinaldo Alessandrini), Complesso Barocco (Alan Curtis), Ensemble Aurora (Enrico Gatti),  Ensemble Baroque de Nice (Gilbert Bezzina), Le Parlement de Musique (Martin Gester), Akâdèmia (Francoise Lasserre), Modo Antiquo (F.M.Sardelli), Ensemble Elyma (Gabriel Garrido), La Risonanaza (Fabio Bonizzoni) and La Venexiana (Claudio Cavina) further afield.

She has performed, in prestigious  festivals, opera houses and concert halls in the USA, South America, Japan, Israel and throughout Europe. She has frequently broadcast for European radio stations, including Radio France, RAI, BBC, WDR, ÖRF, RTSI.

She has recorded numerous CDs for well known labels (Opus 111, Glossa, Arcana, Tactus), obtaining many international prizes, such as the Gramophone Award, Diapason d’Or, Prix Cecilia, Preis der deutschen Schallplattenkritik, Palmares des Palmares, Cannes Classical Award, Grand Prix du Disque, Premio Vivaldi della Fondazione Cini (Venice).

In 2009, together with her fellow singers Giuseppe Maletto and Daniele Carnovich, she founded the ensemble La Compagnia del Madrigale. They record madrigals of the Renaissance for Glossa (Madrid).

Ms Bertini teaches opera singing at the Accademia Musicale Alta Valdera (Pisa) and Renaissance and Baroque singing at Pistoia Musica Antica.